LIPA-α-syn aggregates (red) from COS-7 cells (purple) are binding and accumulating early endosomes (green)
(Confocal Leica TCS SP8-STED Microscope, deconvoluted with Huygens Software)
Skills and Expertise
Bacterial culture: Escherichia coli (12 differents strains, 6 pathogenic P2 level), Lactobacillus rhamnosus (11 differents strains).
Biochemical assays: Western Blot (SDS-PAGE, NuPAGE; LI-COR machine), Dot blot (Filter retardation assay), Co-immunoprecipitation.
Biomolecular techniques: PCR (cloning, screening of contaminants), qPCR.
Immortalized cell lines: BV2, Caco-2, COS-7, E.G7-OVA, HEK 293T, Hep G2, HeLa, IMG, Neuro-2a, PC-12, SH-SY5Y.
iPSC cells: human iPSC Ngn2-inducible, patient-derived Dopaminergic Neurons, iPSC-derived glutamatergic neurons (iNeurons), iPSC-derived dopaminergic neurons.
Cell transfection: Calcium phosphate, Lipofection (Lipofectamine 2000/3000, ViaFect, FastFect), Nucleofection, PEI.
Oxidative stress readouts: CellRox Green/Red (Thermofisher), DCFDA (Abcam), Mitosox (Thermofisher), tBARS (RD Systems).
Cell-death readouts: CellEvent caspase3/7 (Thermofisher), Annexin V/PI (Thermofisher), LDH release (Thermofisher).
Quality controls: Mycoplasma detection kits (ATCC).
Immunocytochemistry: One to four colors staining in immortalized or iPSC cell lines.
Lentivirus: Production of 3rd generation lentivirus, infection and creation of stable cell lines.
Confocal microscopy: LSM 770 (Carl Zeiss, fixed samples), LSM 780 (Carl Zeiss, fixed samples), Leica DMI-8 STED 3X (Leica, Fixed samples, live-cell imaging - Super resolution), Quorum Wave FX Spinning Disc (Live-cell imaging)
Epifluorescent microscopy: Axio observer with apotome (Carl Zeiss, live-cell imaging)
Fields of expertise: Alpha-synuclein, Neurology, Microbiology, Oxidative Stress, Parkinson's disease, Protein aggregation, Protein misfolding, Endolysosomal system, Endosomes, Lysosomes.
Bibliographic tools: Zotero.
Biomolecular tool: SnapGene
FACS software: FlowJo.
Imaging post-treatment: Adobe photoshop (Adobe), Adobe illustrator (Adobe), Powerpoint (Office), Fiji (ImageJ).
Imaging acquisition/analysis software:
Microscope software: ZEN (Zeiss), Leica Application Suite X (LAS X, Leica)
Deconvolution: Huygens Software - Deconvolution module (Scientific Volume Imaging)
Image analysis: Fiji (ImageJ), Image Studio Lite (LI-COR)
3D analysis: Imaris Software - Spots and volume analyses, surface rendering (Oxford instruments)
Deep learning segmentation tools: WEKA, Ilastik
Office suite: Excel, Powerpoint, Word (Office).
Statistic software: GraphPad Prism (GraphPad Software), Excel (Office).
Others: I'm very flexible, open-minded and a fast self-learner when I need to use and master a new software.